Protocols for Cell culture

All the insights and materials you need to run robust cell culture experiments in clear, step-by-step protocols designed by our experts and partners.

• BMDC isolation protocol This protocol will guide you through isolating immature bone marrow-derived dendritic cells (BMDCs) from mice.
• Cell culture and maintenance protocol Cells require careful maintenance to prevent contamination, facilitate growth and ensure long-term stability. The following pages provide general guidance for preserving, thawing and maintaining cultured cells. Note: specific procedures will be highly dependent on the cell line used, and you should refer to the cell line providers for detailed advice.
• Counting cells using a haemocytometer View our detailed step-by-step protocol explaining how to obtain a viable cell count from a hemocytometer.
• Cryopreservation of mammalian cell lines video protocol Learn about cryopreservation of mammalian cell lines for long term storage in liquid nitrogen.
• Differentiation of 3t3-L1 cells into adipocyte-like cells This protocol outlines how to chemically induce the differentiation of 3T3-L1 cells into adipocyte-like cells, adapted from Reed & Lane (1980).
• Induction of apoptosis in cells Procedure for biological and chemical induction of apoptosis in cells.
• Isolation of human platelets from whole blood This protocol describes the isolation of human platelets from whole blood.
• RNA isolation and reverse transcription Find out the procedure for RNA isolation, DNase treatment and reverse transcription in our detailed protocol for cells in culture and tissue samples.